gephryin synaptic systems 147 011c3 antibody Search Results


post synaptic inhibitory marker gephryin  (Synaptic Systems)
90
Synaptic Systems post synaptic inhibitory marker gephryin
Post Synaptic Inhibitory Marker Gephryin, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gephryin+synaptic+systems+147+011c3+antibody/147+011C3/pmc08508811-156-57-61
Average 90 stars, based on 1 article reviews
post synaptic inhibitory marker gephryin - by Bioz Stars, 2026-10
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pre synaptic inhibitory marker vesicular gaba transporter vgat  (Synaptic Systems)
96
Synaptic Systems pre synaptic inhibitory marker vesicular gaba transporter vgat
Acute, but not chronic, fluoxetine alters <t>inhibitory</t> synapse formation. ( A ) Schematic of inhibitory pre- <t>(VGAT-positive)</t> and post- (gephyrin-positive) synaptic compartments. Image created using lab-licensed Biorender account. ( B ) 3-month-old cortical spheroids were either acutely or chronically treated with 1.5 μg/mL fluoxetine. 10 μm-thick cryosections were immunostained for inhibitory synapse markers, pre-synaptic VGAT (red) and post-synaptic gephyrin (green) and were imaged using confocal microscopy. Scale bar = 10, 100 μm. ( C – H ) Individual pre- (VGAT+) and post- (gephyrin+) synaptic markers, as well as co-localization between pre- and post-synaptic markers were analyzed to determine the size and density of inhibitory synapses per cryosection. A threshold of 0.001 synapses per μm 2 DAPI was used for inclusion in the study. n = 12 cryosections from 4 independent cortical spheroid cultures for the untreated control, 9 cryosections from 3 independent cortical spheroid cultures for acute FLX, and 9 cryosections from 3 independent cortical spheroid cultures for chronic FLX. * p < 0.05, One-way ANOVA.
Pre Synaptic Inhibitory Marker Vesicular Gaba Transporter Vgat, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gephryin+synaptic+systems+147+011c3+antibody/131+004/pmc08508811-156-43-50
Average 96 stars, based on 1 article reviews
pre synaptic inhibitory marker vesicular gaba transporter vgat - by Bioz Stars, 2026-10
96/100 stars
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post synaptic excitatory marker post synaptic density protein 95 psd 95  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology post synaptic excitatory marker post synaptic density protein 95 psd 95
Acute, but not chronic, fluoxetine alters <t>inhibitory</t> synapse formation. ( A ) Schematic of inhibitory pre- <t>(VGAT-positive)</t> and post- (gephyrin-positive) synaptic compartments. Image created using lab-licensed Biorender account. ( B ) 3-month-old cortical spheroids were either acutely or chronically treated with 1.5 μg/mL fluoxetine. 10 μm-thick cryosections were immunostained for inhibitory synapse markers, pre-synaptic VGAT (red) and post-synaptic gephyrin (green) and were imaged using confocal microscopy. Scale bar = 10, 100 μm. ( C – H ) Individual pre- (VGAT+) and post- (gephyrin+) synaptic markers, as well as co-localization between pre- and post-synaptic markers were analyzed to determine the size and density of inhibitory synapses per cryosection. A threshold of 0.001 synapses per μm 2 DAPI was used for inclusion in the study. n = 12 cryosections from 4 independent cortical spheroid cultures for the untreated control, 9 cryosections from 3 independent cortical spheroid cultures for acute FLX, and 9 cryosections from 3 independent cortical spheroid cultures for chronic FLX. * p < 0.05, One-way ANOVA.
Post Synaptic Excitatory Marker Post Synaptic Density Protein 95 Psd 95, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gephryin+synaptic+systems+147+011c3+antibody/PSD-95+Antibody/pmc08508811-156-25-34
Average 94 stars, based on 1 article reviews
post synaptic excitatory marker post synaptic density protein 95 psd 95 - by Bioz Stars, 2026-10
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pre synaptic excitatory marker vesicular glutamate transporter 1 vglut1  (Synaptic Systems)
97
Synaptic Systems pre synaptic excitatory marker vesicular glutamate transporter 1 vglut1
Expression of serotonin receptors in human cortical spheroids. ( A ) 3-month-old untreated cortical spheroids were immunostained for the serotonin receptor, 5-HT2A (red), and excitatory post- (PSD95, blue) and pre- <t>(VGLUT1,</t> magenta) synaptic markers and imaged with confocal microscopy together with endogenous β-actin-GFP. The same cryosection is shown for comparison of 5-HT2A association with PSD95 and VGLUT1. Co-localization between 5-HT2A and the respective synaptic marker is shown in grayscale. The same region of interest is enlarged for the merged and co-localized images. ( B ) Analysis of 5-HT2A colocalization with pre- and post-synaptic markers. ~20% of 5-HT2A co-localizes with pre-synaptic VGLUT1. n = 11 cryosections from 4 independent cortical spheroid cultures. ( C ) 3-month-old untreated cortical spheroids were immunostained for the serotonin receptor, 5-HT5A (magenta), and doublecortin (blue) to identify neurons and GFAP (red) to identify astrocytes and were imaged with confocal microscopy together with endogenous β-actin-GFP. The same cryosection is shown for comparison of 5-HT5A association with doublecortin (DCX) and GFAP. Co-localization between 5-HT5A and either DCX-positive neurons or GFAP-positive astrocytes is shown in grayscale. The same region of interest is enlarged for the merged and co-localized images. ( D ) Analysis of DCX-positive neurons or GFAP-positive astrocytes co-localized with 5-HT5A. Approximately 30% of GFAP co-localizes with 5-HT5A, significantly more than the ~20% of DCX positive neurons. n = 12 cryosections from 4 independent cortical spheroid cultures. Scale bar = 100, 20 μm.
Pre Synaptic Excitatory Marker Vesicular Glutamate Transporter 1 Vglut1, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gephryin+synaptic+systems+147+011c3+antibody/135+304/pmc08508811-156-9-17
Average 97 stars, based on 1 article reviews
pre synaptic excitatory marker vesicular glutamate transporter 1 vglut1 - by Bioz Stars, 2026-10
97/100 stars
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Image Search Results


Acute, but not chronic, fluoxetine alters inhibitory synapse formation. ( A ) Schematic of inhibitory pre- (VGAT-positive) and post- (gephyrin-positive) synaptic compartments. Image created using lab-licensed Biorender account. ( B ) 3-month-old cortical spheroids were either acutely or chronically treated with 1.5 μg/mL fluoxetine. 10 μm-thick cryosections were immunostained for inhibitory synapse markers, pre-synaptic VGAT (red) and post-synaptic gephyrin (green) and were imaged using confocal microscopy. Scale bar = 10, 100 μm. ( C – H ) Individual pre- (VGAT+) and post- (gephyrin+) synaptic markers, as well as co-localization between pre- and post-synaptic markers were analyzed to determine the size and density of inhibitory synapses per cryosection. A threshold of 0.001 synapses per μm 2 DAPI was used for inclusion in the study. n = 12 cryosections from 4 independent cortical spheroid cultures for the untreated control, 9 cryosections from 3 independent cortical spheroid cultures for acute FLX, and 9 cryosections from 3 independent cortical spheroid cultures for chronic FLX. * p < 0.05, One-way ANOVA.

Journal: International Journal of Molecular Sciences

Article Title: Effects of the Selective Serotonin Reuptake Inhibitor Fluoxetine on Developing Neural Circuits in a Model of the Human Fetal Cortex

doi: 10.3390/ijms221910457

Figure Lengend Snippet: Acute, but not chronic, fluoxetine alters inhibitory synapse formation. ( A ) Schematic of inhibitory pre- (VGAT-positive) and post- (gephyrin-positive) synaptic compartments. Image created using lab-licensed Biorender account. ( B ) 3-month-old cortical spheroids were either acutely or chronically treated with 1.5 μg/mL fluoxetine. 10 μm-thick cryosections were immunostained for inhibitory synapse markers, pre-synaptic VGAT (red) and post-synaptic gephyrin (green) and were imaged using confocal microscopy. Scale bar = 10, 100 μm. ( C – H ) Individual pre- (VGAT+) and post- (gephyrin+) synaptic markers, as well as co-localization between pre- and post-synaptic markers were analyzed to determine the size and density of inhibitory synapses per cryosection. A threshold of 0.001 synapses per μm 2 DAPI was used for inclusion in the study. n = 12 cryosections from 4 independent cortical spheroid cultures for the untreated control, 9 cryosections from 3 independent cortical spheroid cultures for acute FLX, and 9 cryosections from 3 independent cortical spheroid cultures for chronic FLX. * p < 0.05, One-way ANOVA.

Article Snippet: The primary antibodies used in this experiment included the pre-synaptic excitatory marker vesicular glutamate transporter 1 (VGLUT1) (Synaptic Systems Goettingen, Germany 135 304, 1:1000), the post-synaptic excitatory marker post synaptic density protein 95 (PSD-95) (Santa Cruz Biotechnology Inc, Dallsa, TX, sc-32291, 1:50), the pre-synaptic inhibitory marker vesicular GABA transporter (VGAT) (Synaptic Systems 131 004, 1:1000), and the post-synaptic inhibitory marker gephryin (Synaptic Systems 147 011C3, 1:500).

Techniques: Confocal Microscopy, Control

Expression of serotonin receptors in human cortical spheroids. ( A ) 3-month-old untreated cortical spheroids were immunostained for the serotonin receptor, 5-HT2A (red), and excitatory post- (PSD95, blue) and pre- (VGLUT1, magenta) synaptic markers and imaged with confocal microscopy together with endogenous β-actin-GFP. The same cryosection is shown for comparison of 5-HT2A association with PSD95 and VGLUT1. Co-localization between 5-HT2A and the respective synaptic marker is shown in grayscale. The same region of interest is enlarged for the merged and co-localized images. ( B ) Analysis of 5-HT2A colocalization with pre- and post-synaptic markers. ~20% of 5-HT2A co-localizes with pre-synaptic VGLUT1. n = 11 cryosections from 4 independent cortical spheroid cultures. ( C ) 3-month-old untreated cortical spheroids were immunostained for the serotonin receptor, 5-HT5A (magenta), and doublecortin (blue) to identify neurons and GFAP (red) to identify astrocytes and were imaged with confocal microscopy together with endogenous β-actin-GFP. The same cryosection is shown for comparison of 5-HT5A association with doublecortin (DCX) and GFAP. Co-localization between 5-HT5A and either DCX-positive neurons or GFAP-positive astrocytes is shown in grayscale. The same region of interest is enlarged for the merged and co-localized images. ( D ) Analysis of DCX-positive neurons or GFAP-positive astrocytes co-localized with 5-HT5A. Approximately 30% of GFAP co-localizes with 5-HT5A, significantly more than the ~20% of DCX positive neurons. n = 12 cryosections from 4 independent cortical spheroid cultures. Scale bar = 100, 20 μm.

Journal: International Journal of Molecular Sciences

Article Title: Effects of the Selective Serotonin Reuptake Inhibitor Fluoxetine on Developing Neural Circuits in a Model of the Human Fetal Cortex

doi: 10.3390/ijms221910457

Figure Lengend Snippet: Expression of serotonin receptors in human cortical spheroids. ( A ) 3-month-old untreated cortical spheroids were immunostained for the serotonin receptor, 5-HT2A (red), and excitatory post- (PSD95, blue) and pre- (VGLUT1, magenta) synaptic markers and imaged with confocal microscopy together with endogenous β-actin-GFP. The same cryosection is shown for comparison of 5-HT2A association with PSD95 and VGLUT1. Co-localization between 5-HT2A and the respective synaptic marker is shown in grayscale. The same region of interest is enlarged for the merged and co-localized images. ( B ) Analysis of 5-HT2A colocalization with pre- and post-synaptic markers. ~20% of 5-HT2A co-localizes with pre-synaptic VGLUT1. n = 11 cryosections from 4 independent cortical spheroid cultures. ( C ) 3-month-old untreated cortical spheroids were immunostained for the serotonin receptor, 5-HT5A (magenta), and doublecortin (blue) to identify neurons and GFAP (red) to identify astrocytes and were imaged with confocal microscopy together with endogenous β-actin-GFP. The same cryosection is shown for comparison of 5-HT5A association with doublecortin (DCX) and GFAP. Co-localization between 5-HT5A and either DCX-positive neurons or GFAP-positive astrocytes is shown in grayscale. The same region of interest is enlarged for the merged and co-localized images. ( D ) Analysis of DCX-positive neurons or GFAP-positive astrocytes co-localized with 5-HT5A. Approximately 30% of GFAP co-localizes with 5-HT5A, significantly more than the ~20% of DCX positive neurons. n = 12 cryosections from 4 independent cortical spheroid cultures. Scale bar = 100, 20 μm.

Article Snippet: The primary antibodies used in this experiment included the pre-synaptic excitatory marker vesicular glutamate transporter 1 (VGLUT1) (Synaptic Systems Goettingen, Germany 135 304, 1:1000), the post-synaptic excitatory marker post synaptic density protein 95 (PSD-95) (Santa Cruz Biotechnology Inc, Dallsa, TX, sc-32291, 1:50), the pre-synaptic inhibitory marker vesicular GABA transporter (VGAT) (Synaptic Systems 131 004, 1:1000), and the post-synaptic inhibitory marker gephryin (Synaptic Systems 147 011C3, 1:500).

Techniques: Expressing, Confocal Microscopy, Comparison, Marker

Fluoxetine does not alter excitatory synapse formation. ( A ) 3-month-old cortical spheroids were either acutely or chronically treated with 1.5 μg/mL fluoxetine. 10 μm-thick cryosections were immunostained for excitatory synapse markers, pre-synaptic VGLUT1 (magenta) and post-synaptic PSD95 (blue). Synapses were imaged together with endogenous β-actin-GFP (green) using confocal microscopy. Scale bar = 100 μm. ( B – D ) Co-localization between pre- and post-synaptic markers was analyzed to determine the size and density of excitatory synapses per cryosection. A threshold of 0.001 synapses per μm 2 DAPI was used for inclusion in the study. n = 9 cryosection regions from 4 independent cortical spheroid cultures for the untreated control, 7 cryosection regions from 3 independent cortical spheroid cultures for acute FLX, and 9 cryosection regions from 3 independent cortical spheroid cultures for chronic FLX. * p < 0.05, One-way ANOVA.

Journal: International Journal of Molecular Sciences

Article Title: Effects of the Selective Serotonin Reuptake Inhibitor Fluoxetine on Developing Neural Circuits in a Model of the Human Fetal Cortex

doi: 10.3390/ijms221910457

Figure Lengend Snippet: Fluoxetine does not alter excitatory synapse formation. ( A ) 3-month-old cortical spheroids were either acutely or chronically treated with 1.5 μg/mL fluoxetine. 10 μm-thick cryosections were immunostained for excitatory synapse markers, pre-synaptic VGLUT1 (magenta) and post-synaptic PSD95 (blue). Synapses were imaged together with endogenous β-actin-GFP (green) using confocal microscopy. Scale bar = 100 μm. ( B – D ) Co-localization between pre- and post-synaptic markers was analyzed to determine the size and density of excitatory synapses per cryosection. A threshold of 0.001 synapses per μm 2 DAPI was used for inclusion in the study. n = 9 cryosection regions from 4 independent cortical spheroid cultures for the untreated control, 7 cryosection regions from 3 independent cortical spheroid cultures for acute FLX, and 9 cryosection regions from 3 independent cortical spheroid cultures for chronic FLX. * p < 0.05, One-way ANOVA.

Article Snippet: The primary antibodies used in this experiment included the pre-synaptic excitatory marker vesicular glutamate transporter 1 (VGLUT1) (Synaptic Systems Goettingen, Germany 135 304, 1:1000), the post-synaptic excitatory marker post synaptic density protein 95 (PSD-95) (Santa Cruz Biotechnology Inc, Dallsa, TX, sc-32291, 1:50), the pre-synaptic inhibitory marker vesicular GABA transporter (VGAT) (Synaptic Systems 131 004, 1:1000), and the post-synaptic inhibitory marker gephryin (Synaptic Systems 147 011C3, 1:500).

Techniques: Confocal Microscopy, Control